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| '''Complete Series 1:''' Count and examine phenotypes of autosomal vs. X-linked crosses; <BR> '''Start Series 2: Classical (Forward) Genetics- Part 1- Perform mutant hunt:''' <BR> Pick (3) putative Dpy mutants to separate plates | | '''Complete Series 1:''' Count and examine phenotypes of autosomal vs. X-linked crosses; <BR> '''Start Series 2: Classical (Forward) Genetics- Part 1- Perform mutant hunt:''' <BR> Pick (3) putative Dpy mutants to separate plates | ||
|'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate) | |'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate) | ||
| '''Homework:''' Read ALL of Series 2:background information [[BISC_219/F10:_Gene_Mapping_Info]] and about all work to be performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our experimental question & goals. Outline the experimental process | | '''Homework:''' Read ALL of Series 2:background information [[BISC_219/F10:_Gene_Mapping_Info]] and about all work to be performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our overall topic and experimental question(s) & goals. Outline the experimental process. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Assignment explained and rubric found at [[BISC_219/F10: Assignment Series2_Outline_Summary]]<BR> | ||
Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]]; <BR> Read the journal article, Indentification of Genes that Regulate a Left-Right Aymmetric Neuronal Migration in ''Caenorhabditis elegans'' published in ''Genetics'' 164: 1355-1367 (August 2003), for discussion in lab next time. Full text of article found at: [http://www.genetics.org/cgi/content/full/164/4/1355]; See [[BISC_219/F10:Questions to Guide Your Reading1]] for information on how to prepare for this discussion | Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]]; <BR> Read the journal article, Indentification of Genes that Regulate a Left-Right Aymmetric Neuronal Migration in ''Caenorhabditis elegans'' published in ''Genetics'' 164: 1355-1367 (August 2003), for discussion in lab next time. Full text of article found at: [http://www.genetics.org/cgi/content/full/164/4/1355]; See [[BISC_219/F10:Questions to Guide Your Reading1]] for information on how to prepare for this discussion | ||
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