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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 06:04, 20 September 2010
, 20 September 2010→BISC219 F10 Weekly Lab Planner
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| '''Complete Series 1:''' Count and examine phenotypes of autosomal vs. X-linked crosses; <BR> '''Start Series 2: Classical (Forward) Genetics- Part 1- Perform mutant hunt:''' <BR> Pick (3) putative Dpy mutants to separate plates | | '''Complete Series 1:''' Count and examine phenotypes of autosomal vs. X-linked crosses; <BR> '''Start Series 2: Classical (Forward) Genetics- Part 1- Perform mutant hunt:''' <BR> Pick (3) putative Dpy mutants to separate plates | ||
|'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate) | |'''3 days after lab''':Examine mutants from hunt: check phenotype <BR>– if Dpy then cross L4 mutant hermaphrodites by L4 N2 males<BR> (2 plates – duplicate) | ||
| '''Homework:''' Read ALL of Series 2:background information and all work performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our experimental question & goals. Outline the experimental process, including all parts of the experimental design: Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Assignment explained and rubric found at [[BISC_219/F10: Assignment Series2_Outline_Summary]]<BR> | | '''Homework:''' Read ALL of Series 2:background information [[BISC_219/F10:_Gene_Mapping_Info]] and about all work to be performed in Labs 2-6 on our Classical (Forward) Genetics project. Write a summary of our experimental question & goals. Outline the experimental process, including all parts of the experimental design: Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. Use this outline to write a 1-2 page summary of how you will determine the exact location and extent of the gene and protein change and include the significance of the mutation in worms and, if possible, broader significance in other species. Assignment explained and rubric found at [[BISC_219/F10: Assignment Series2_Outline_Summary]]<BR> | ||
Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]]; <BR> Read the journal article, Indentification of Genes that Regulate a Left-Right Aymmetric Neuronal Migration in ''Caenorhabditis elegans'' published in ''Genetics'' 164: 1355-1367 (August 2003), for discussion in lab next time. Full text of article found at: [http://www.genetics.org/cgi/content/full/164/4/1355]; See [[BISC_219/F10:Questions to Guide Your Reading1]] for information on how to prepare for this discussion | Start Data Analysis (Results) of your autosomal vs. X-linked testing DUE at the beginning of LAB 4. <BR>Grading rubric & Assignment info at:<BR> [[BISC_219/F10: Assignment Help- Data Analysis 1]]; <BR> Read the journal article, Indentification of Genes that Regulate a Left-Right Aymmetric Neuronal Migration in ''Caenorhabditis elegans'' published in ''Genetics'' 164: 1355-1367 (August 2003), for discussion in lab next time. Full text of article found at: [http://www.genetics.org/cgi/content/full/164/4/1355]; See [[BISC_219/F10:Questions to Guide Your Reading1]] for information on how to prepare for this discussion | ||
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