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BISC 219/F10:Calendars/Planner: Difference between revisions
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→BISC219 F10 Weekly Lab Planner
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| Sept. 7 to <br>Sept. 13 | | Sept. 7 to <br>Sept. 13 | ||
| '''LEARN WORM HUSBANDRY;''' <BR>View worm videos;<BR> Make your worm pick; <BR>Examine worms: recognize different stages & sexes & differentiate mutations;<BR> Practice picking worms;<BR>'''Start Series 1: Set up autosomal and X-linked crosses''' | | '''LEARN WORM HUSBANDRY;''' <BR>View worm videos;<BR> Make your worm pick; <BR>Examine worms: recognize different stages & sexes & differentiate mutations;<BR> Practice picking worms;<BR>'''Start Series 1: Set up autosomal and X-linked crosses''' | ||
|'''3 days after lab:''' pick (2) wild type worms from each cross to new plates (3 plates total);<br>Examine the phenotypes of the progeny – hermaphrodites and males | |'''3 days after lab:''' pick (2) wild type worms from each cross to new plates (3 plates total);<br>Examine the phenotypes of the progeny – hermaphrodites and males - record all information in your notebook | ||
| '''Homework''':Complete Entry Survey; Familiarize yourself with the information in the [[BISC_219/F10:Resources]] section;<br>Read all of the background information on ''C. elegans'' found in [[BISC_219/F10:_Worm_Info]] and read the Lab 1 and Lab 2 information about our first Series 1: Autosomal vs. Sex-Linked Inheritance at [[BISC_219/F10:_Gene_Linkage]] & [[BISC_219/F10:_Lab_2]]; Complete Graded Assignment 1 explained at [[BISC_219/F10: Assignment_1_Lab1]] | | '''Homework''':Complete Entry Survey; Familiarize yourself with the information in the [[BISC_219/F10:Resources]] section;<br>Read all of the background information on ''C. elegans'' found in [[BISC_219/F10:_Worm_Info]] and read the Lab 1 and Lab 2 information about our first Series 1: Autosomal vs. Sex-Linked Inheritance at [[BISC_219/F10:_Gene_Linkage]] & [[BISC_219/F10:_Lab_2]]; Complete Graded Assignment 1 explained at [[BISC_219/F10: Assignment_1_Lab1]] | ||
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! 3 | ! 3 | ||
| Sept. 21 to <br> Sept. 27 | | Sept. 21 to <br> Sept. 27 | ||
| '''Series 2- Part 2: Linkage Analysis''': cross males from last cross to the (5) test strains (5 plates total);<BR> Journal article discussion | | '''Series 2- Part 2: Linkage Analysis''': cross heterozygous males (+/d) from last cross to the (5) test strains (5 plates total);<BR> Journal article discussion | ||
| '''3 days after lab: Linkage:''' transfer (2) L4 hermaphrodites from each cross<br> to new plates (5 plates total) | | '''3 days after lab: Linkage:''' transfer (2) L4 hermaphrodites from each cross<br> to new plates (5 plates total) | ||
| '''Homework: '''Draw crosses and diagram strategy for Series 2 Linkage Analysis due at the beginning of Lab 4. Information and grading rubric found at [[BISC_219/F10: Assignment_Series2_Linkage Testing Crosses]]<BR> | | '''Homework: '''Draw crosses and diagram strategy for Series 2 Linkage Analysis due at the beginning of Lab 4. Information and grading rubric found at [[BISC_219/F10: Assignment_Series2_Linkage Testing Crosses]]<BR> | ||
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! 5 | ! 5 | ||
| Oct. 5 to<br> Oct. 12 | | Oct. 5 to<br> Oct. 12 | ||
| '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains ( | | '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>;'''3 days after lab:''' '''Series2: Mapping:''' pick ( | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>;'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross to individual plates (4 plates total);<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your dpy mutation. Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | | '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your dpy mutation. Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | ||
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