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BISC 219/F10:Calendars/Planner: Difference between revisions
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! 6 | ! 6 | ||
| Oct. 13 to <br> Oct. 19 | | Oct. 13 to <br> Oct. 19 | ||
| '''Series2:Mapping:''' SCORE!; <BR> '''Series3: Reverse Genetics:'''Restriction enzyme digest PCR product/clean-up/ligation into pPD129.36 vector/transformation into BL21 ''E.coli'' | | '''Series2:Mapping:''' SCORE!; <BR> '''Series2:Mapping: Gene Sequence Analysis''' '''Series3: Reverse Genetics:'''Restriction enzyme digest PCR product/clean-up/ligation into pPD129.36 vector/transformation into BL21 ''E.coli'' | ||
| Determine map distance between your mutant gene <BR>and the known reference mutation. <BR>Check control & transformation plates (save) – <BR>notify instructor if no colonies | | Determine map distance between your mutant gene <BR>and the known reference mutation. <BR>Check control & transformation plates (save) – <BR>notify instructor if no colonies | ||
| '''Homework''': DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at [[BISC_219/F10: Assignment_Series2_DNA Sequencing]]<BR> | | '''Homework''': DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at [[BISC_219/F10: Assignment_Series2_DNA Sequencing]]<BR> | ||
