DNA extraction - Salting Out: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
no edit summary
No edit summary
Line 77: Line 77:
==Discussion==
==Discussion==
*I have noticed in some samples a top layer following addition of the salt that looks like protein or fat (white and fluffy) but does not precipitate with the salt.  I always pipette from beneath this layer to remove the aqeous phase.  If anyone knows more about this or has a way to make it precipitate with the cell debris, please make a note here.
*I have noticed in some samples a top layer following addition of the salt that looks like protein or fat (white and fluffy) but does not precipitate with the salt.  I always pipette from beneath this layer to remove the aqeous phase.  If anyone knows more about this or has a way to make it precipitate with the cell debris, please make a note here.
==BioStream version==
Following is the DNA extraction - Salting Out protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream (see Source code). More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth|Vaishnavi]]
====Text Output====
[[DNA extraction - Salting Out protocol]]
====Source Code====
[[DNA extraction - Salting Out - source code]]


You can [[Talk:{{PAGENAME}}|discuss this protocol]].  
You can [[Talk:{{PAGENAME}}|discuss this protocol]].  

Navigation menu