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BISC 219/2009: Mod 1 C. elegans General Information: Difference between revisions
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BISC 219/2009: Mod 1 C. elegans General Information (view source)
Revision as of 07:19, 1 September 2009
, 1 September 2009→Nematode Genetics
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The majority of the mutants characterized to date are non-lethal and display a clearly visible phenotype. A large number of mutants are "uncoordinated" (now representing nearly 100 genes). Uncoordinated phenotypes range from small aberrations in movement to nearly complete paralysis. There are some great movies of hermaphrodite, male and mutant movement at [http://130.15.90.245/c__elegans_movies.htm Dr. Ian Chin-Sang's website]. | The majority of the mutants characterized to date are non-lethal and display a clearly visible phenotype. A large number of mutants are "uncoordinated" (now representing nearly 100 genes). Uncoordinated phenotypes range from small aberrations in movement to nearly complete paralysis. There are some great movies of hermaphrodite, male and mutant movement at [http://130.15.90.245/c__elegans_movies.htm Dr. Ian Chin-Sang's website]. | ||
Morphological mutants include dumpy, small, long, and blistered animals. Dumpy mutants are shorter than wild-type animals and correspond to twenty different genes dispersed over the six | Morphological mutants include dumpy, small, long, and blistered animals. Dumpy mutants are shorter than wild-type animals and correspond to twenty different genes dispersed over the six chromosomes and are useful for mapping most other classes of mutants, since the double mutants are easily distinguished. Both morphological and uncoordinated mutants can be used for mapping many types of developmental mutants. | ||
An increasing variety of mutations are now being studied including those affecting drug-resistance, chemotaxis, thermotaxis, male sexual behavior, catabolic pathways, dopamine biosynthesis, muscle assembly, sex determination, development of the ventral nerve cord, and temperature-sensitive lethal mutants affecting embryogenesis and gonadogenesis. | An increasing variety of mutations are now being studied including those affecting drug-resistance, chemotaxis, thermotaxis, male sexual behavior, catabolic pathways, dopamine biosynthesis, muscle assembly, sex determination, development of the ventral nerve cord, and temperature-sensitive lethal mutants affecting embryogenesis and gonadogenesis. | ||
Maintaining genetic stocks of mutants and wild type strains is less difficult with ''C. elegans'' than with some other organisms used in developmental or behavioral studies, such as ''Drosophila'' or the mouse. Stocks of ''C. elegans'' remain viable when frozen and stored in liquid nitrogen. | Maintaining genetic stocks of mutants and wild type strains is less difficult with ''C. elegans'' than with some other organisms used in developmental or behavioral studies, such as ''Drosophila'' or the mouse. Stocks of ''C. elegans'' remain viable when frozen and stored in liquid nitrogen. | ||
