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LacZ staining of cells: Difference between revisions
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The bacterial enzyme β-galactosidase (gene LacZ) is frequently used as reporter gene. It can be easily located with a LacZ stain using the artificial substrate [[X-gal]], which turns blue when it is cleaved by β-galactosidase. | The bacterial enzyme β-galactosidase (gene LacZ) is frequently used as reporter gene. It can be easily located with a LacZ stain using the artificial substrate [[X-gal]], which turns blue when it is cleaved by [[β-galactosidase]]. | ||
This protocol describes the staining of cultured cells containing active LacZ genes. | This protocol describes the staining of cultured cells containing active LacZ genes. | ||
