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LacZ staining of cells: Difference between revisions
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{{back to protocols}} | {{back to protocols}} | ||
The bacterial enzyme β-galactosidase (gene LacZ) is frequently used as reporter gene. It can be easily located with a LacZ stain using the artificial substrate X-gal. | The bacterial enzyme β-galactosidase (gene LacZ) is frequently used as reporter gene. It can be easily located with a LacZ stain using the artificial substrate [[X-gal]], which turns blue when it is cleaved by β-galactosidase. | ||
This protocol | This protocol describes the staining of cultured cells containing active LacZ genes. | ||
== material == | == material == | ||
