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Annealing complementary primers: Difference between revisions
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''See also [[Annealing | ''See also [[Annealing primers]]'' | ||
==Introduction== | ==Introduction== | ||
A simple and cheap way to make a short (< 100 bp) piece of DNA is to order two complementary primers from a company such as [http://www.invitrogen.com Invitrogen]. Other options are described under [[Synthetic Biology:BioBricks/Part fabrication | part fabrication]] and [[Annealing and primer extension|annealing and primer extension]]. Having annealed the primers, you most likely want to use them as an insert into a [[Synthetic Biology:Vectors | vector]] (the linked page describes BioBrick vectors). Here are some pages that describe methods to assemble two parts if they correspond to a BioBricks standard - | A simple and cheap way to make a short (< 100 bp) piece of DNA is to order two complementary primers from a company such as [http://www.invitrogen.com Invitrogen]. Other options are described under [[Synthetic Biology:BioBricks/Part fabrication | part fabrication]] and [[Annealing and primer extension|annealing and primer extension]]. Having annealed the primers, you most likely want to use them as an insert into a [[Synthetic Biology:Vectors | vector]] (the linked page describes BioBrick vectors). Here are some pages that describe methods to assemble two parts if they correspond to a BioBricks standard - | ||
