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LacZ staining of cells: Difference between revisions
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* X-gal stock is 200 mg/ml in dimethylformamide (DMF) | * X-gal stock is 200 mg/ml in dimethylformamide (DMF) | ||
* K ferri-cyanide ( 50mM ) = | * K ferri-cyanide ( 50mM ) =Fe<sup>3-</sup> is 0,33 g / 20 ml PBS ( Sigma Cat.# P-8131) | ||
* K ferro-cyanide ( 50mM ) = | * K ferro-cyanide ( 50mM ) =Fe<sup>4-</sup> is 0,42 g / 20 ml PBS ( Sigma Cat.# P-9387) | ||
=== staining solution === | === staining solution === | ||
