User contributions for Sean E. Lauber
From OpenWetWare
Jump to navigationJump to search
5 November 2013
- 13:5613:56, 5 November 2013 diff hist +118 Sean Lauber:MTT Cell Proliferation Assay No edit summary current
16 September 2013
- 13:1813:18, 16 September 2013 diff hist +102 Sean Lauber:AMIRA - Density Overlays No edit summary current
- 13:0613:06, 16 September 2013 diff hist +73 Sean Lauber:ELISA Development No edit summary current
12 September 2013
- 10:0810:08, 12 September 2013 diff hist −211 Sean Lauber:MTT Cell Proliferation Assay No edit summary
- 10:0110:01, 12 September 2013 diff hist +4 Sean Lauber:Adenovirus infection (in vitro) No edit summary current
13 August 2013
- 06:5806:58, 13 August 2013 diff hist −137 Sean Lauber:Cytokine Stimulation of Cells No edit summary current
6 August 2013
- 07:2107:21, 6 August 2013 diff hist 0 File:Adenovirus infection in vitro.xlsx uploaded a new version of "Image:Adenovirus infection in vitro.xlsx" current
29 July 2013
- 06:3106:31, 29 July 2013 diff hist +323 Sean Lauber:MTT Cell Proliferation Assay No edit summary
22 July 2013
- 08:3208:32, 22 July 2013 diff hist +186 Sean Lauber:MTT Cell Proliferation Assay No edit summary
- 08:0908:09, 22 July 2013 diff hist +113 User:Sean E. Lauber →Template excel files (always download the current file) current
- 08:0808:08, 22 July 2013 diff hist 0 N File:Adenovirus infection in vitro.xlsx No edit summary
- 07:4807:48, 22 July 2013 diff hist +379 Sean Lauber:Adenovirus infection (in vitro) No edit summary
- 07:0007:00, 22 July 2013 diff hist +28 User:Sean E. Lauber →Template excel files (always download the current file)
- 06:5906:59, 22 July 2013 diff hist +33 User:Sean E. Lauber →Template excel files (always download the current file)
- 06:5906:59, 22 July 2013 diff hist 0 N File:IV injection of cells calculator.xlsx No edit summary current
- 06:5906:59, 22 July 2013 diff hist 0 N File:Cell seeding calculator.xlsx No edit summary current
- 06:5706:57, 22 July 2013 diff hist +38 User:Sean E. Lauber →Template excel files (always download the current file)
- 06:5606:56, 22 July 2013 diff hist 0 N File:Cell seeding and injection of cells calculator.xlsx No edit summary current
19 July 2013
- 07:1407:14, 19 July 2013 diff hist −96 Sean Lauber:Isotype ELISA against Adenovirus No edit summary current
- 06:5906:59, 19 July 2013 diff hist +122 Sean Lauber:Isotype ELISA against Adenovirus No edit summary
- 06:2506:25, 19 July 2013 diff hist +2 Sean Lauber:PCR No edit summary current
- 06:2406:24, 19 July 2013 diff hist +425 Sean Lauber:PCR No edit summary
- 06:1206:12, 19 July 2013 diff hist +99 User:Sean E. Lauber →Template excel files (always download the current file)
- 06:1206:12, 19 July 2013 diff hist 0 File:ELISA spiking template.xlsx uploaded a new version of "Image:ELISA spiking template.xlsx" current
- 06:0806:08, 19 July 2013 diff hist 0 N File:B16 or LLC injection template.xlsx No edit summary current
- 06:0706:07, 19 July 2013 diff hist +8 Sean Lauber:mOSM ELISA No edit summary current
- 06:0506:05, 19 July 2013 diff hist +54 Sean Lauber:mOSM ELISA No edit summary
- 06:0406:04, 19 July 2013 diff hist +2 Sean Lauber:mOSM ELISA No edit summary
- 06:0406:04, 19 July 2013 diff hist +1,482 N Sean Lauber:mOSM ELISA New page: This protocol requires the special primary antibody in a box in the fridge. This protocol uses reagent diluent from R&D, but you can use sterile filtered 1% BSA (I have tested this and it ...
- 05:5805:58, 19 July 2013 diff hist +40 User:Sean E. Lauber →Molecular Techniques
18 July 2013
- 13:4913:49, 18 July 2013 diff hist +2,043 N Sean Lauber:Arginase assay New page: This procedure is meant to look for arginase activity in macrophages. The higher this activity is, it's believed that this is more of an M2 macrophage. Cells from the BAL can first be adhe... current
- 13:4113:41, 18 July 2013 diff hist +48 User:Sean E. Lauber →Molecular Techniques
- 13:3913:39, 18 July 2013 diff hist +286 Sean Lauber:Preparing LLC or B16 cells for injection No edit summary current
- 13:1213:12, 18 July 2013 diff hist +1,888 N Sean Lauber:Preparing LLC or B16 cells for injection New page: NOTE that if you are using LLC-Fluc cells, you MUST culture the cells with 3 ug/ml of puromycin (in the same box with fungizone). For every 10 ml of media, add 6 ul of the puromycin soluti...
- 12:5412:54, 18 July 2013 diff hist +99 User:Sean E. Lauber →Animal Work
- 12:5212:52, 18 July 2013 diff hist +74 User:Sean E. Lauber →Animal Work
- 12:4912:49, 18 July 2013 diff hist −21 User:Sean E. Lauber →Animal Work
- 12:4912:49, 18 July 2013 diff hist +284 Sean Lauber:Using the Kras G12D Mouse Model for Lung Cancer No edit summary current
- 09:2209:22, 18 July 2013 diff hist +222 N Sean Lauber:Using the Kras G12D Mouse Model for Lung Cancer New page: This procedure involves first precipitating adenovirus before it is administered by intubation. For 1000 ul: CaCl2 2 ul 10X EMEM 100 ul 1. Calculate the appr...
- 09:0409:04, 18 July 2013 diff hist +3 Sean Lauber:Culturing of Primary Macrophages No edit summary current
- 09:0109:01, 18 July 2013 diff hist +74 Sean Lauber:Peritoneal Lavage No edit summary current
- 08:5908:59, 18 July 2013 diff hist +764 N Sean Lauber:Peritoneal Lavage New page: If you're doing this to culture the cells then you want to use sterile practices! Otherwise, simply perform it outside of the TC hood. 1. Anesthetize a mouse and squirt 70% EtOH on its a...
- 08:5108:51, 18 July 2013 diff hist +613 Sean Lauber:Broncho-alveolar Lavage No edit summary current
- 08:4108:41, 18 July 2013 diff hist −42 User:Sean E. Lauber →Animal Work
- 08:3808:38, 18 July 2013 diff hist +976 N Sean Lauber:Formalin Fixation & Preparation for Histology New page: This procedure involves fixing of lung tissue for 48 hours in 10% formalin before moving to 70% EtOH. 1. Perfuse your lungs with ample 10% formalin before placing in a labeled 15 ml falc... current
- 08:2108:21, 18 July 2013 diff hist −2 Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer No edit summary current
- 08:1808:18, 18 July 2013 diff hist +1,444 N Sean Lauber:AMIRA - Using Snagit New page: Download Snagit from here: http://www.oldapps.com/SnagIT.php?old_snagit=12. Apparently it's free with a key in the comments section. Versions after 7.2.5 aren't free. Unfortunately I can'... current
- 08:0408:04, 18 July 2013 diff hist +56 User:Sean E. Lauber →Technical
- 08:0208:02, 18 July 2013 diff hist 0 Sean Lauber:AMIRA - Density Overlays No edit summary
- 08:0108:01, 18 July 2013 diff hist 0 N File:Densityoverlay 07.png No edit summary current
