SBB09 protocols

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All these protocols in one big list

Polymerase Reactions

Wobble Reaction
Cloning by PCR
SOEing PCR

Cleanup Reactions

Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions

EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations

Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Assays

Growth rate determination
Streptavidin-binding assay
Silver biomineralization assay
Cell-cell adhesion assay

Other

LR Gateway Transfers
What do I do with my sequencing data?

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  • This page was last edited on 6 June 2009, at 00:42.
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