Protocols/Nucleic acids

General

• Nucleic acid precipitation
• Phenol/chloroform extraction

DNA

• Agarose gel electrophoresis
• Annealing Primers
• Designing primers
• DNA Ligation
• Library Generation
• Miniprep
• PCR
• Phosphatase treatment of linearized vector
• Polony Protocols (Church/Mitra Lab)
• PNK Treatment of DNA Ends
• Purification of DNA
• Reconstituting primers
• Restriction digest
• 'Round-the-horn site-directed mutagenesis
• Sequencing DNA
• Site-directed mutagenesis
• Templiphi

RNA

• In vitro transcription with T7 RNA polymerase
• Northern Blot, ³²P End-Labeled Probes
• Probe Prep, ³²P End-Labeled Probes
• RNA Extraction
• RNase Protection Assay