Protocols/Nucleic acids

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General

  • Nucleic acid precipitation
  • Phenol/chloroform extraction

DNA

  • Agarose gel electrophoresis
  • Annealing Primers
  • Designing primers
  • DNA Ligation
  • Library Generation
  • Miniprep
  • PCR
  • Phosphatase treatment of linearized vector
  • Polony Protocols (Church/Mitra Lab)
  • PNK Treatment of DNA Ends
  • Purification of DNA
  • Reconstituting primers
  • Restriction digest
  • 'Round-the-horn site-directed mutagenesis
  • Sequencing DNA
  • Site-directed mutagenesis
  • Templiphi


RNA

  • In vitro transcription with T7 RNA polymerase
  • Northern Blot, 32P End-Labeled Probes
  • Probe Prep, 32P End-Labeled Probes
  • RNA Extraction
  • RNase Protection Assay
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Protocols/Nucleic_acids&oldid=20969"

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  • This page was last edited on 16 February 2006, at 13:04.
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