Protocols/Escherichia coli

• Bacterial cell culture
• Bacterial Transformation
  • Preparing chemically competent cells (TSS)
  • Transforming chemically competent cells (TSS)
  • Preparing electrocompetent cells
  • Transforming electrocompetent cells
• β-Galactosidase Assay (A better Miller)
• Colony PCR
• Making a long term stock of bacteria
• Miniprep
• P1vir phage transduction
• Pouring plates
• Protein expression resource
• Chemostat Protocol