Paulsson:Pmalkus papers
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Plasmids
- Thomas CM. Transcription regulatory circuits in bacterial plasmids. Biochem Soc Trans. 2006 Dec;34(Pt 6):1072-4. DOI:10.1042/BST0341072 |
- Boye E, Stokke T, Kleckner N, and Skarstad K. Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12206-11. DOI:10.1073/pnas.93.22.12206 |
- Womble DD and Rownd RH. Regulation of mini-F plasmid DNA replication. A quantitative model for control of plasmid mini-F replication in the bacterial cell division cycle. J Mol Biol. 1987 May 5;195(1):99-113. DOI:10.1016/0022-2836(87)90330-5 |
- Kornacki JA and Firshein W. Replication of plasmid RK2 in vitro by a DNA-membrane complex: evidence for initiation of replication and its coupling to transcription and translation. J Bacteriol. 1986 Jul;167(1):319-26. DOI:10.1128/jb.167.1.319-326.1986 |
- Gerdes K, Rasmussen PB, and Molin S. Unique type of plasmid maintenance function: postsegregational killing of plasmid-free cells. Proc Natl Acad Sci U S A. 1986 May;83(10):3116-20. DOI:10.1073/pnas.83.10.3116 |
- Karunakaran P, Blatny JM, Ertesvåg H, and Valla S. Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression. J Bacteriol. 1998 Aug;180(15):3793-8. DOI:10.1128/JB.180.15.3793-3798.1998 |
- Blasina A, Kittell BL, Toukdarian AE, and Helinski DR. Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3559-64. DOI:10.1073/pnas.93.8.3559 |
- Firshein W and Kim P. Plasmid replication and partition in Escherichia coli: is the cell membrane the key?. Mol Microbiol. 1997 Jan;23(1):1-10. DOI:10.1046/j.1365-2958.1997.2061569.x |
- Zzaman S, Abhyankar MM, and Bastia D. Reconstitution of F factor DNA replication in vitro with purified proteins. J Biol Chem. 2004 Apr 23;279(17):17404-10. DOI:10.1074/jbc.M400021200 |
- Conley DL and Cohen SN. Isolation and characterization of plasmid mutations that enable partitioning of pSC101 replicons lacking the partition (par) locus. J Bacteriol. 1995 Feb;177(4):1086-9. DOI:10.1128/jb.177.4.1086-1089.1995 |
- Miller C and Cohen SN. Separate roles of Escherichia coli replication proteins in synthesis and partitioning of pSC101 plasmid DNA. J Bacteriol. 1999 Dec;181(24):7552-7. DOI:10.1128/JB.181.24.7552-7557.1999 |
- Kim PD, Rosche TM, and Firshein W. Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2. Plasmid. 2000 May;43(3):214-22. DOI:10.1006/plas.2000.1467 |
Partitioning
- Ho TQ, Zhong Z, Aung S, and Pogliano J. Compatible bacterial plasmids are targeted to independent cellular locations in Escherichia coli. EMBO J. 2002 Apr 2;21(7):1864-72. DOI:10.1093/emboj/21.7.1864 |
Use FISH to simultaneously analyze localization of F, P1, and RK2 plasmids (from separate incompatibility groups). Find that they are spatially segregated.
- Pogliano J, Ho TQ, Zhong Z, and Helinski DR. Multicopy plasmids are clustered and localized in Escherichia coli. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4486-91. DOI:10.1073/pnas.081075798 |
RK2 and pUC19 localization using lacO/GFP-LacI system. Plasmids usually in just a few foci at center or quater positions. Number of foci increases with cell length and growth rate.
- Lau IF, Filipe SR, Søballe B, Økstad OA, Barre FX, and Sherratt DJ. Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol Microbiol. 2003 Aug;49(3):731-43. DOI:10.1046/j.1365-2958.2003.03640.x |
Generated lacO and teO arrays with random inter-operator sequences.
- Møller-Jensen J, Borch J, Dam M, Jensen RB, Roepstorff P, and Gerdes K. Bacterial mitosis: ParM of plasmid R1 moves plasmid DNA by an actin-like insertional polymerization mechanism. Mol Cell. 2003 Dec;12(6):1477-87. DOI:10.1016/s1097-2765(03)00451-9 |
Plasmid R1 partitioning by F-actin like filaments formed by ParM
- Møller-Jensen J, Jensen RB, Löwe J, and Gerdes K. Prokaryotic DNA segregation by an actin-like filament. EMBO J. 2002 Jun 17;21(12):3119-27. DOI:10.1093/emboj/cdf320 |
Fluorescent proteins
- Griesbeck O, Baird GS, Campbell RE, Zacharias DA, and Tsien RY. Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J Biol Chem. 2001 Aug 3;276(31):29188-94. DOI:10.1074/jbc.M102815200 |