Paulsson:Facs

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Where to do it

Systems Biology FACS facility (accessible through SysBio Intranet [1])

talk to Tricia Rogers

No bacteria in the (Aria) sorter!

CBR

requires PO, looser rules, several sorters

How to bleach prior to fixation?

test in plate reader with live cells

using scope lamps/lasers?

Post-arrest maturation?

on ice

cholamphenicol

NaN3

Chloride sensitivity (media?)

Biotech and Bioeng v25 no10 p2485

deg-tag vs. photobleach... which ahs better signal strength