McClean:Kanamycin media

Overview

Protocol for making kanamycin plates. This is based on addgene's antibiotic recommendations

Materials

1. LB1. See McClean:LB1
2. Kanamycin (50mg/ml) (1000x) stock solution in the -20C chest in 3007. See McClean:Chloramphenicol

Procedure

• Melt the LB1 in the microwave if it is solid. Start by microwaving it for 2 minutes, and then add 1 minutes intervals until it is liquid.
• Place the hot, liquid LB1 onto a shaker. This will let it cool without letting it solidify.
• When the LB1 is no longer painfully hot to the touch, add 100μL of kanamycin stock solution for every 100mL of LB1 for a final working concentration of 50μg/mL of kanamycin.
• Place the LB1+ kNmycin solution back on a shaker to for a little bit to ensure that it is well-mixed.
• Pour out the plates.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

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Contact

*Cameron J. Stewart: April 19, 2016

or instead, discuss this protocol.