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LB agar media


  • Bacto tryptone
  • Bacto yeast extract
  • NaCl
  • Bacto Agar


  • Begin with approximately 800mls of MiliQ water
  • Weigh out and add ingredients with constant stirring:
    • Bacto tryptone 10g
    • Bacto yeast extract 5g
    • NaCl 5g
    • Bacto Agar 15g
  • Bring up to 1000mls with MiliQ water
  • Separate into two 1L bottoles of 500mls each
  • Autoclave


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

*Megan N McClean 13:24, 2 July 2015 (EDT) Many LB formulations call for twice as much NaCl (10g instead of 5g in this protocol).



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