McClean:Chloramphenicol

Overview

Protocol for making 20 ml of 1360x (stringent) stock solution of chloramphenicol (the concentration of this stock is also 200x of the "relaxed" working concentration from Endy link, though it's not clear why this "relaxed" version is useful). This was adapted from Endy:Preparing_Antibiotic_Stocks. See also Chloramphenicol

CAUTION: Chloramphenicol is a suspected carcinogen. Use gloves, goggles, and the fume hood.

Materials

1. 200 proof ethanol (100%)
2. chloramphenicol (BP904-100)

Procedure

• Fill a 50mL centrifuge tube with 20mL of 200 proof ethanol. (we use a centrifuge tube because it must withstand vigorous vortexing)
• Weigh out 680 mg chloramphenicol. Insert this into the tube.
• Vortex the tube vigorously until it is well-mixed.
• Aliquot the solution into 1.5mL eppendorf tubes. Label each tube. Store them in a labelled bag in the -20C chest freezer in 3007.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

Contact

*Cameron J. Stewart: April 19, 2016

or instead, discuss this protocol.