Manalis:Back Door

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Lab equipment

• Admin Inventory Record (PDF)
  • Inventory Record (XLSX, 2 sheets)

• Manalis:Camera

• Manalis:Wirebonder
  • Bonding tool gauge part number: 3475.013 (20$) from Westbond
  • Useful contact: David Lesperance 714 978 1551 at Westbond

• • Good bonding tips:
    • MCSOE-1/16-828-45-C-2025-MP (suitable for Al and Au wire)
    • TCSOE-1/16-828-45-C-2025-MP (suitable for Al wire)

• Media Lab Machine Shop
  • Manalis:Modela
  • MediaLab Lasercutter Tutorial
    • You can order acrylic in bulk from J.Freeman
  • [Waterjet Tutorial (Andrea's comments in blue)]
    • Get the software from \\hub\winhub\cba\Omax\old CD (?)
• Edgerton Machine Shop
• Coulter Counter
  • [Download Background Info]
  • Dan 774-249-4478 daberger@beckman.com

DataSheets

• Particles

New Inventory Chemicals

Chemical Name	Physical State	Amount	Unit	Location
Percoll (Sigma P1644)	LIQUID	0.25	LITER	Fridge

-80degC Yeast Strains

In Amon lab: 4th freezer from left, 3rd row down, 3rd case over (Alexi's). Box labeled: Andrea-Manalis Lab YPJx is in the s288c WT background and from Paul Jorgensen

Shorthand	Details
Emr WT	SEY 6210 (MATalpha, leu2-3, 112ura3-52his3-delta200trp1-delta901lys2-801suc2-delta9)
Emr Fab1d	fab1delta is replaced with HIS
YPJ5	WT control for YPJ9+10
YPJ9	Cln3d::LEU2
YPJ10	whi5d::natR
YPJ12	BUD21-YFP-his5+, SEC63-CFP-KanR (control for YPJ14)
YPJ14	sfp1d::URA3, BUD21-YFP-his5+, SEC63-CFP-KanR
F44,5	prototrophic WT diploid

Tissue Culture

• [Cell Growth Media Recipes]

Other Information

• [Old Wiring Diagram]
• [Wesley's DrC Diagram]
• Manalis:Cadence
• YEP+glu media = ~900mL sterile 1.05x liquid YEP media + 50mL 40% glu stock + 10mL 100x adenine stock

Administrative

• [Vacation Reporting Form]
• Also, upload planned travel on the google calendar: calendar@media.mit.edu

Publication Responsibilities

• For some time now, the NIH has required that all publications that were supported by their money be deposited in PubMed Central, where they are freely available. It is the authors' responsibility to ensure that this occurs:

• http://publicaccess.nih.gov/index.htm [1]