Love:Protocols

Common Buffers and Blocking Solutions

• PBS 10x stock solution

Buffers for Processing Protein Microarrays

• Blocking solution stock for Antibody Arrays- PBS/3%BSA
• Washing solution stock - PBS/1% Tween 20 (PBST 1%)

Tissue Culture Media

• General DMEM media for hybridomas (DMEM-10)
• Cell fusion-Myeloma partner cells
• Cloning media for single cells (DMEM-20+HCF)

Mammalian Cell Culture

General Procedures

• Freezing cells for storage in LN2

Hybridomas

• Cell fusion-Myeloma partner cells
• Cell Fusion-Fusion and Selection Medias

others

• A20

Immunization

• Mice immunization
• test bleed + elisa

Probes

• Peptide Probe Conjugation (streptavidin)
• Peptide Probe Conjugation (KLH)

Soft Lithography

General Procedures

• PDMS pouring
• Extract unreacted monomer
• Plasma cleaning
• Preparing poly-lysine slides

Microengraving (μEn)

• Genepix
• μEn hybridoma screening
• Counting

Others

Autoclave

• Dry Run
• Wet Run
• Biohazardous Waste