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For sterilizing tissue culture waste. Autoclave room is on the fourth floor of E25, adjacent to the freight elevator. Note: Use clear autoclave-safe bags and not the red biohazardous bags.

  • Set autoclaving conditions:
    • Press Dry button.
    • Sterilization time: 60-120 minutes (small to large).
    • Drying time: 10 minutes.
  • Add 1 liter of water into bag for better sterilization.
  • Loosely tie the waste bag with safety tag. Fill in the information on the tag.
  • Put waste in autoclavable secondary container.
  • Place container in the autoclave.
  • Close the autoclave door, turn the latch, and tighten the wheel.
  • Press Start.
  • Ensure the temperature reaches 121°C before leaving.
  • Enter information into the log.
  • When cycle is complete, make sure the pink strip on the safety tag has become black.
  • Throw the waste in normal trash bin.