Klapperich Lab:Notebook/Lab Meeting Notes/2009/04/02

Project name

Main project page Previous entry      Next entry

2 April 2009 Lab Meeting Pressure sensor - quoted $325 SP70A or $1000+ SP70D, returning old one for them to look Co. says that it works fine. Transducer up to 1000 psi. We are getting it back. STATUS? † Announcements Need posters for Oakridge soon. † Flu R01 † SEPSIS Project Submitted to LOC. †RNA project Need to get Jurkat RNA to Jeff. He may visit in May. † COBRA Integration update? Let's get some of this done before JD leaves for Korea. Substrates (JZ) Let's get this to a point where we can publish and move on....JZ and CMK to discuss. - nanopatterned COP provides access to repeatable morphology - Engineered COP substrate? UPDATE? three categories of making new sbustrates 1) ebeam COP film, 2) COP hot eboss EVAP (Luca), 3)stamping/other intermediate method(Bjoern similar). (JD) Colloid experiment. - Do Rhodamine. ordered -Need a size specific positive control. PS beads? charged? 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate co-evaporating diluted PS beads didn't give a signal: difficulty in locating concentrated particles † Valve Array/Valve building - done. † Fraunhofer: LOAC Paper submitted to LOC † Biointerfaces group Cathie in charge of Bionterfaces Paper1. Cathie has final draft now before it goes to collaborators. designing herringbone groove for better trapping investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force). † CIMIT- Colson Grant IRB still in revision. † PCR Cathie read and gave back PCR 1 paper to QQ and MCK Qingqing has written the introduction repeated test of the PCR efficiency for different designs of channel. test PCR efficiency using smaller DNA size (63bp product) analyze PCR product for different temperature using bioanalyzer. will make figures for PCR1 paper. developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) † RCA/HDA Cathie submitted One pager Whitepaper. Waiting for response. convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE? Preliminary no-valve design for 25 microliters. 20 more test chips to Sonali (AACC poster). Sonali try to do 10 microliters in the tube † Senior project Megan Test on Thu, Fri worked but failure after some cycles. Need to enhance bonding (e.g. Ar or O2 ashing) † F31: Cochlea † Silica Optimization (Lambda): The following will be submitted monday total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm. Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm . Chip ran with total of 5ng of NA , pico green using the low range standard curve