Klapperich Lab:Notebook/Lab Meeting Notes/2009/04/09

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9 April 2009 Lab Meeting

† Announcements

  • Need posters for Oakridge by Wed. morning.

† Flu R01

  • Sonali is working on RTPCR and plaque assays for progress report.

† SEPSIS Project

  • Submitted to LOC.

†RNA project

  • Need to get Jurkat RNA to Jeff. He may visit in May.


  • Integration with the glass slide.
  • Prototype next Wednesday.
  • Get PMMA film.

  • Substrates (JZ)
    • Let's get this to a point where we can publish and move on....JZ and CMK to discuss.
  • Colloid experiment.

- Do Rhodamine. Leaked. Need to try again.

    • ordered

-Need a size specific positive control. PS beads? charged?

    • 1um unmodified, undiluted PS beads give distinctive spectrum on Ranjith's substrate
    • try coated PS beads.

† Valve Array/Valve building - done.

† Fraunhofer: LOAC

  • Paper submitted to LOC
  • Bonding meeting with MIT folks - Alex left some samples with them.

† Biointerfaces group

  • Cathie in charge of Bionterfaces Paper1. Cathie has final draft now before it goes to collaborators.
  • designing herringbone groove for better trapping
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).

† CIMIT- Colson Grant

  • IRB still in revision.


  • Cathie read and gave back PCR 1 paper to QQ and MCK
  • Qingqing has written the introduction
  • repeated test of the PCR efficiency for different designs of channel.
  • test PCR efficiency using smaller DNA size (63bp product)
  • analyze PCR product for different temperature using bioanalyzer.
  • will make figures for PCR1 paper.
  • developing large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)


  • Cathie submitted One pager Whitepaper. Waiting for response.
  • convert this whitepaper to grant? RCA/HDA on a chip for NIH...anyone interested? ANYONE?
  • Preliminary no-valve design for 25 microliters.
  • 20 more test chips to Sonali (AACC poster).
  • Sonali try to do 10 microliters in the tube

† Senior project

  • Megan
  • HDA on chip. Make simple chip that mixes two things for HDA. Move chip to heater, do HDA demonstration = home run.
  • Plasma helped bonding.
  • EHS wants MSDS stuff.

† F31: Cochlea

† Silica Optimization (Lambda):

  • The following will be submitted monday

  • total of 20 channels 10 as negative and 10 positive with 100ng total NA mass. Emission spectrum of negative samples with excitation at 488nm.
  • Emission spectrum of negative samples at excitations corresponding to the NA sampler kit dyes
  • Emission spectrum for the positive samples split into 2 groups , one with pico green and the other without at 488nm .
  • Chip ran with total of 5ng of NA , pico green using the low range standard curve