Kafatos:Protocols

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Lab specific protocols

  • Anopheles gambiae dsRNA gene KD and midgut dissection after malaria infection
  • Competent Cells
  • dsRNA Production by PCR
  • Extraction of Beauveria bassiana fungal spores
  • Instructions for the culture of Anopheles gambiae cell lines
  • totalRNA extraction from adult mosquitoes
  • totalRNA extraction from cells
  • genomic DNA extraction from individual mosquitoes
  • Minipreps
  • His-tagged Protein Purification (denaturing)
  • Making 1kb+ DNA Ladder Stock
  • Waste handling in the P3

Protocol template


  • Parasite Protocols of the Billker Lab.
  • Imperial College health and safety department


Protocol collections on the web

  • a repository of OWW shared protocols
  • Nature Protocols
  • Protocol Online - Your lab's reference book
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Kafatos:Protocols&oldid=366749"

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  • This page was last edited on 13 November 2009, at 07:52.
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