User:Jhdavis

I recently joined the Sauer Lab. clpX holla!!!!

Protocols

1. Western Blots by Eyal Western::Eyal
2. GFP Purification by organic extraction GFPOrganic
3. Expression/Prep of ClpXdN-linked Trimers ClpXPrep
4. Peptide cleavage PeptideCleavage
5. Flp recombinase Flping
6. ClpP his prep from Kim 2000 ClpPHisPrep
7. Sumo Protease expression/purification from Malakhov 2004 SumoPrep
8. regenerating qiagen columns regenQiagen
9. atpase notes atpaseNotes
10. Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Immunoprecip

Buffers

1. PD Buffer
2. ATP Regeneration Mix
3. YEG-Cl
4. Sauer:Davis Minimal Media
5. 4x reducing SDS-PAGE loading buffer

Info

• GelFiltrationStandards
• MetCleavage
• lacIoperators
• DNA 1 letter codes
• Useful pymol
• A260:A280 DNA:Protein ratios
• fplc singup

Links

1. Promoter standard
2. novagen plasmid compatibility

Sheets

1. File:Asdfsa.xlsx