Jimenez-gomez lab:stock antibiotics
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| abb | stock conc. | LB plate conc. |
Vol. in 500ml LB agar |
MS plate conc |
Vol. in 1L MS | |
|---|---|---|---|---|---|---|
| ampicilin | ap | 50 mg/ml | 100 μg/ml | 1 ml | ||
| kanamycin | km/kn | 50 mg/ml | 50 μg/ml | 0.5 ml | 50 μg/ml | 1 ml |
| spetinomycin | sp | 100 mg/ml | 100 μg/ml | 0.5 ml | ||
| chloramphenicol | cm | 34 mg/ml in ethanol | 34 μg/ml | 0.5 ml | ||
| rifampicin | rif | 34 mg/ml in methanol | 150 μg/ml | 2.2 ml | ||
| tetracycline | tc | 12 mg/ml | 12 μg/ml | 0.5 ml | ||
| hygromycin | hyg | 20 mg/ml | 20 μg/ml | 1 ml | ||
| carbenicillin | carb | 100 mg/ml | 500 μg/ml | 5 ml | ||
| thiamine | 50 mg/ml | 50 μg/ml | 1 ml | |||
| gentamycin* | gent | effective dose 6.5 mg/ml | 15 μg/ml | 0.75 ml | 75 μg/ml | 1 ml |
| basta | 20 mg/ml | 20 μg/ml | 1 ml | |||
| zeocin | Zeo | 100 mg/ml | 25 μg/ml | 0.125 ml | ||
| * the potency of gentamycin varies from stock to stock, the volume added must be calculated according to |- | ||||||
| For pPZP211 Spectinomycin Dihydrochloride and Streptomycin sulfate disolved in dd water with a final concentration of 30 mg/ml each. Take 1 ul for each mL of medium (final concentration of 30 ug/mL. | ||||||
