Jimenez-gomez lab:stock antibiotics

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Institute Jean Pierre Bourgin
INRA - Versailles
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Antibiotic stocks

abb stock conc. LB plate
conc.
Vol. in 500ml
LB agar
MS plate
conc
Vol. in
1L MS
ampicilin ap 50 mg/ml 100 μg/ml 1 ml
kanamycin km/kn 50 mg/ml 50 μg/ml 0.5 ml 50 μg/ml 1 ml
spetinomycin sp 100 mg/ml 100 μg/ml 0.5 ml
chloramphenicol cm 34 mg/ml in ethanol 34 μg/ml 0.5 ml
rifampicin rif 34 mg/ml in methanol 150 μg/ml 2.2 ml
tetracycline tc 12 mg/ml 12 μg/ml 0.5 ml
hygromycin hyg 20 mg/ml 20 μg/ml 1 ml
carbenicillin carb 100 mg/ml 500 μg/ml 5 ml
thiamine 50 mg/ml 50 μg/ml 1 ml
gentamycin* gent effective dose 6.5 mg/ml 15 μg/ml 0.75 ml 75 μg/ml 1 ml
basta 20 mg/ml 20 μg/ml 1 ml
zeocin Zeo 100 mg/ml 25 μg/ml 0.125 ml
* the potency of gentamycin varies from stock to stock, the volume added must be calculated according to |-
For pPZP211 Spectinomycin Dihydrochloride and Streptomycin sulfate disolved in dd water with a final concentration of 30 mg/ml each. Take 1 ul for each mL of medium (final concentration of 30 ug/mL.