IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/02/19
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February 19th 2010
Constructing the systems and put them together.
-What are the sites for restriction enzymes in the operon?
-Can we change the color expression?
This part is complemented whit information exposed in operón Lux. We are trying to together these systems.
-What else is regulated by Ompr?
-Search for more information of Ompr.
-What are the phenotypes of Envz?
There is a trouble trying to activate the LovTAP operon. We can resolve them trying with Tryptophan auxotroph or a Tryptophan repressor knockout.
-Know about mutants phentoypes.
There is an advantage whit this bioluminescence system because LW of light can active one of the purposed phytochrome.
-Can it be applied in E. Coli?
-What is the best option for Ca +2 influx?
-How can be regulated Ompr?
-Search in literature for mutants phenotypes.
-Search for their paths and viability.
-Search in literature how much light can be emitted by bioluminescent molecules.
-Know all the parameters.