IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/06/10
From OpenWetWare
Jump to navigationJump to search
iGEM Project | Main project page Previous entry Next entry |
Transformation of the PBBRMCS-5 and the PCR amplification of PSB1T3 ligation into DH5αThe ligation of PBBRMCS-5 and the PCR amplification PSB1T3 transformed into E. coli DH5α Transformation1 tube with 100 μL of competent E. coli DH5α cells with 8 μL of the PBBRMCS-5 and PSB1T3 ligation 1 tube with 50 μL of competent E. coli DH5α cells with no DNA as a control.
Incubate over night and check for red colonies the next day.
|