IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/06/10
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Transformation of the PBBRMCS-5 and the PCR amplification of PSB1T3 ligation into DH5α
The ligation of PBBRMCS-5 and the PCR amplification PSB1T3 transformed into E. coli DH5α
1 tube with 100 μL of competent E. coli DH5α cells with 8 μL of the PBBRMCS-5 and PSB1T3 ligation 1 tube with 50 μL of competent E. coli DH5α cells with no DNA as a control.
Incubate over night and check for red colonies the next day.