IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/06/23
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Regrowing PLasmid PBBRMCS-5 in E. coli BL21-DE3
2 tubes with 5 mL of liquid LB and 5 μL of gentamicin with E. coli BL21-DE3 containing the Plasmid PBBRMCS-5.
1 tube with 5 mL of liquid LB and 5 μL of gentamicin as a control.
Leave for 12 hours and the extract the plasmid.