IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/06/07

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Blunting Ends

We blunted the sticky ends of the DNA digestion

Blunting

  • NEBuffer 2 10X -> 2μL
  • dNTPs -> 4 μL
  • Digested PBBRMCS-5 Plasmid DNA -> 10 μL
  • Klenow -> 1μL

For a total reaction of 20 μL.

Leave for 1 hour at 25ºC.

Stop Knlenow actitivy at 75ºC during 20 minutes.