IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/02

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Thursday 2nd September The overnight digestions were checked using gel electrophoresis [[1]]. The gel was set up as follows starting from the bottom: 1) GFP plasmid from the lab. 2) Part BBa_E0040. 3) Ladder. Both bands in lane 2 were removed using the gel extraction kit.[[2]] * Ligation of pgaA promoter, Part BBa_E0040 and pSB1C3: pgaA promoter - 2μL Part BBa_E0040 - 2μL pSB1C3 - 2μL T4 DNA ligase - 1μL T4 buffer (x10) - 2μL H2O - 11μL Ligation failed as shown by the bellow gel: The ligation is in lane one. The three bands show that ligations was not successful.