IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/01

iGEM Project name 1

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Wednesday 1st September Carried out gel electrophoresis [[1]] and gel extraction[[2]] of the overnight ligations: The gel was set up accordingly, with lane one starting from the top: 1) Ladder. 2) Part BBa_E0040. 3) csrB Promoter. 4) pga Promoter. 5) pSB1C3. 6) pACYC. 7) pACYC. The short bands from lanes 7,6,5,4 and 1 were extracted using the gel extraction kit. Overnight restriction digests were set up as shown bellow: 1) GFP from lab in unknown plasmid: Plasmid - 10μL Xba1 - 2μL Pst1 - 2μL Buffer 3 - 2μL BSA (x20) - 1μL 2) Part BBa_E0040 (GFP) Plasmid - 10μL Xba1 - 2μL Pst1 - 3μL Buffer 3 - 2μL BSA (x20) - 1μL