IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/01

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Wednesday 1st September

  • Carried out gel electrophoresis [[1]] and gel extraction[[2]] of the overnight ligations:
  • The gel was set up accordingly, with lane one starting from the top:

1) Ladder. 2) Part BBa_E0040. 3) csrB Promoter. 4) pga Promoter. 5) pSB1C3. 6) pACYC. 7) pACYC.


UVP00444.JPG

  • The short bands from lanes 7,6,5,4 and 1 were extracted using the gel extraction kit.


  • Overnight restriction digests were set up as shown bellow:

1) GFP from lab in unknown plasmid:

  Plasmid - 10μL
  Xba1 - 2μL
  Pst1 - 2μL
  Buffer 3 - 2μL
  BSA (x20) - 1μL

2) Part BBa_E0040 (GFP)

  Plasmid - 10μL
  Xba1 - 2μL
  Pst1 - 3μL
  Buffer 3 - 2μL
  BSA (x20) - 1μL