IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/09/06

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Monday 6th September

  • Multiple overnight restriction digests were set up as shown bellow:

1) Part BBa_E0040:

  Plasmid - 10μL
  Pst1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

2) Part BBa_E0040:

  Plasmid - 10μL
  Xba1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

3) pgaA promoter:

  Plasmid - 10μL
  Spe1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

4) CsrB promoter:

  Plasmid - 10μL
  Spe1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

5) HapR promoter:

  Plasmid - 10μL
  Spe1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

6) Cholera System:

  plasmid - 10μL
  Hind3 - 2μL
  Nco1 - 2μL
  Buffer 2 - 2μL
  H2O - 4μL