IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/31

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Tuesday 31st August:

  • Many overnight restriction digests were set up as shown bellow:

1) pACYC

  Plasmid - 35μL
  Nco1  - 1μL
  Buffer 3 - 4μL
  BSA (x100) - 0.4μL

2) pSB1C3

  Plasmid- 10μL
  Pst1 - 2μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

3) pga Promoter

  Plasmid - 10μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  Spe1 - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

4) csrB

  Plasmid - 10μL
  EcoR1 - 2μL
  EcoR1 buffer - 2μL
  Pst1 - 2μL
  BSA (x20) - 1μL
  H2O - 3μL

5) Part BBa_E0040 (GFP)

  Plasmid - 10μL
  EcoR1 - 2μL
  Spe1 - 2μL
  EcoR1 buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL