IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/26

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Thursday 26th August:

  • Attempted PCR of BarA a second time but again results are gel electrophoresis were poor.
  • Repeated gel electrophoresis [[1]] of the cut pgaA promoter which produced better results:

UVP00428.JPG

  • The now isolated pgaA promoter was extracted from the gel.[[2]]
  • Set up an over night restriction digest of pACYC using Hind3:
  Plasmid- 10μL
  Hind3 - 2μL
  buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL