IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/25

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Wednesday 25th August:

  • Gel electrophoresis[[1]] was carried out on the digested pgaA promoter but the band was too faint to be extracted.
  • Attempted PCR of the BarA gene from DH5-alpha WT.
  • Gel electrophoresis [[2]] was then carried out on the PCR products. However the results shown bellow suggests PCR was unsuccessful.