Tuesday 24th August:
- Transformations failed.
- Ordered competent DH5-alpha e.coli from Bioline.
- Set up an overnight restriction digest of pgaA promoter with EcoR1 and Pst1 restriction enzymes:
Pst1 - 2μL
EcoR1 - 2μL
EcoR1 buffer - 2μL
BSA (x20) - 1μL
H2O - 3μL
- Ran an electrophoresis gel[] of plasmids pACYC duet-1 and pCDF. Results shown bellow:
- The first three wells from the bottom were filled with the cut pACYC duet-1.
- The three wells between the two ladders contain the cut pCDF.
- pACYC duet-1 was extracted from the gel.[]