IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/23

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Monday 23rd August:

  • Transformation of pga promoter was successful.
  • Transformations of GFPs and e. Chromi failed and repeated.
  • Set up an overnight restriction digest of plasmids pACYC-duet, pCDF and pCOLA using Hind3 and Nco1 restiction enzymes:


  Plasmid- 10μL
  Hind3 - 2μL
  Nco1 - 2μL
  buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL