IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/23

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png iGEM Project name 1 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Monday 23rd August:

  • Transformation of pga promoter was successful.
  • Transformations of GFPs and e. Chromi failed and repeated.
  • Set up an overnight restriction digest of plasmids pACYC-duet, pCDF and pCOLA using Hind3 and Nco1 restiction enzymes:

  Plasmid- 10μL
  Hind3 - 2μL
  Nco1 - 2μL
  buffer - 2μL
  BSA (x20) - 1μL
  H2O - 3μL