IGEM:Stanford/2009/Project Homeostasis/Primer/Primersdoc

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Project
Research Proposal
Systems Overview
Cloning Plan
Sequences & Primers
Anti-Inflammation
Device Overview
Parts Design
Challenges
Results
Anti-Immunosuppression
Device Overview
Parts Design
Challenges
Results
Protocols
Modeling
Overview
Notebook
Results
Future Work
Archived Work


Primers Compilation


Device 1:

SoxR (Escherichia coli E24377A)

Forward: TATAgaattcgcggccgcttctagATGGAAAAGAAATTACCCCGC

Reverse: TATAtactagtagcggccgctgcagTTAGTTTTGTTCATCTTCCAGCAAGCGTG


SoxS promoter (Created by Joe 7-6-09)

Forward: TATAgaattcgcggccgcttctagagAATCGCTTTACCTCAAGTTAACTTG

Reverse: TATAtactagtagcggccgctgcagCAGTTCGTTAATTCATCTGTTGGG


Blh (uncultured marine bacterium 66A03)

Forward: TATAgaattcgcggccgcttctagATGGGTCTGATGCTGATTG

Reverse: TATAtactagtagcggccgctgcagTCAGTTTTTGATTTTGATACGGG


RalDH(II) (Rattus Norvegicus*)

Forward: TATAgaattcgcggccgcttctagATGCCCGGCGAGGTGAAGGC

Reverse: TATAtactagtagcggccgctgcagTTAGGAGTTCTTCTGGGGGATC


Device 2:

New IL-6 fusion gene (Created by Joe 7-6-09)

Forward: TATAgaattcgcggccgcttctagATGCCAACAATAACCACTGC

Reverse: TATAtactagtagcggccgctgcagTTATGCTGATGCTGTCAAAG


HlyC (from E. coli str. CFT073)

Forward: TATAgaattcgcggccgcttctagagTTGGTTTGCTTTTTTTTACCTGCCACC

Reverse: TATAtactagtagcggccgctgcagCTCTTAACCTGTTAATG


HlyB (from E. coli str. CFT073)

Forward: TATAgaattcgcggccgcttctagagGGAGTCATAATGGATTCTTGTC

Reverse: TATAtactagtagcggccgctgcagGTTAGTCTGACTGTAACTG


HlyD (from E. coli str. CFT073)

Forward: TATAgaattcgcggccgcttctagATGAAAACATGGTTAATGGGGTTCAGCG

Reverse: TATAtactagtagcggccgctgcagCTCTGAGACTTAACGCTCATG


TolC (from E. coli str. CFT073)

Forward: TATAgaattcgcggccgcttctagagGCAAATGAAGAAATTGCTCC

Reverse: TATAtactagtagcggccgctgcagCGTCATCAGTTACGGAAAGGG


TrpR (E. coli str. IAI1)

Forward: TATAgaattcgcggccgcttctagATGGCCCAACAATCACCCTATTCAGC

Reverse: TATAtactagtagcggccgctgcagTCAATCGCTTTTCAGCAACACCTCTTCCAGCCACTGGC


Trp Promoter and Operator (E. coli: no specified strain )

Forward: TATAgaattcgcggccgcttctagagCTCAAGGCGCACTCCCGTTCTG

Reverse: TATAtactagtagcggccgctgcagTCGATACCCTTTTTACGTGAACTTGCGTACTAG


HlyR: Since HlyR is not a protein. This sequence is sequenced as a chunk with HlyA,HlyB, HlyC. Thus only one primer is needed. primer:

TATAtactagtagcggccgctgcagGAATTCCAAGCGAAGTCC