IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/06

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Théotime
Gel extraction from yesterday

  • Pss/wTet/LuxI (9)
  • Pss/wTet/LuxI (10)
  • Pms/mTet/LuxI (7)
  • Pss/sLac/LuxI (17)
  • Pms/sLac/LuxI (18)


Aleksandra
Ligations for cheracterization : same as 03/09, started with the same digestions, burt once again

  • sTet+Ps
  • mTet+Ps
  • wTet+Ps


Raphaël
Ligations

  • pSB1C3 + Pss/wTet/LuxI (9)
  • pSB1C3 + Pss/sLac/LuxI (17)
  • pSB1C3 + Pms/mTet/LuxI (7)
  • pSB1C3 + Pss/wTet/LuxI (10)
  • pSB1C3 + Pss/sLac/LuxI (18)
  • pSB1C3 ctrl


Transformations in TURBO cells

  • pSB1C3 + Pss/wTet/LuxI (9)
  • pSB1C3 + Pss/sLac/LuxI (17)
  • pSB1C3 + Pms/mTet/LuxI (7)
  • pSB1C3 + Pss/wTet/LuxI (10)
  • pSB1C3 + Pss/sLac/LuxI (18)
  • pSB1C3 ctrl


Overnight cultures

  • sLac/LuxI III
  • LuxI
  • RBS/GFP/tt (E0240)
  • pLux
  • Pss/wTet/LuxI (10), but it didn't grow
  • Pms/mTet/LuxI (7)
  • Pss/sLac/LuxI III (17), but it didn't grow
  • I13507/pLux/GFP
  • attP/Pms/attC/RFP, col 1
  • attP/Psm/attC/RFP, col 1
  • attP/Pss/attC/RFP, col 1
  • Pms/mTet/LuxI chr
  • Pss/wTet/LuxI chr, but it didn't grow
  • pUC chr, but it didn't grow
  • PswTet, colonies 1-4 for characterisation
  • PsmTet, colonies 1-4 for characterisation
  • PssTet, colonies 1-4 for characterisation


Touchdown PCR overnight

  • PswTet, colonies 1-4 for characterisation
  • PsmTet, colonies 1-4 for characterisation
  • PssTet, colonies 1-4 for characterisation


But there was a problem with the PCR machine, it didn't work.