IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/07

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Minipreps

  • sLac/LuxI III
  • LuxI
  • RBS/GFP/tt (E0240)
  • pLux
  • Pms/mTet/LuxI
  • I13507/pLux/GFP
  • PswTet, colonies 1 and 2
  • PsmTet, colonies 1 and 2
  • PssTet, colonies 1 and 2


Restriction digest


With XbaI and PstI

  • LuxI
  • I13507/pLux/GFP


With EcoRI-HF and XbaI

  • RBS/GFP/tt (E0240)


With SpeI and PstI

  • pLux
  • Pms/mTet/LuxI


With EcoRI-HF and SpeI

  • LuxI


With EcoRI-HF and PstI

  • PswTet, colonies 1 and 2
  • PsmTet, colonies 1 and 2
  • PssTet, colonies 1 and 2


Gel electrophoresis

  • LuxI (X+P)
  • LuxI (E+S)
  • I13507/pLux/GFP
  • PswTet, colonies 1
  • PswTet, colonies 2
  • PsmTet, colonies 1
  • PsmTet, colonies 2
  • PssTet, colonies 1
  • PssTet, colonies 2


Gel extraction

  • LuxI (X+P)
  • LuxI (E+S)
  • I13507/pLux/GFP


PCR purification

  • RBS/GFP/tt (E0240)
  • pLux
  • Pms/mTet/LuxI