IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/05

Population counter

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Aleksandra Touchdown PCR of the 13 minipreps from yesterday and also of 6 other constructs (elongation time 6 mins), then gel electrophoresis. Gel 1 (PCR) Minipreps from 31/08 1 Pms/wTet II 2 Pms/mTet III 3 Psm/mTet III 4 Psw/mTet III 5 Pss/wTet II 6 Psw/wTet II Minipreps from yesterday (04/05) 7,8 Pms/mTet/LuxI, 2 cultures 9,10 Pss/wTet/LuxI, 2 cultures 11,12 Psm/sTet/LuxI, 2 cultures 13,14 Psm/wTetIII/I13507/pLux/GFP, 2 cultures 15,16 Psm/wTetII/I13507/pLux/GFP, 2 cultures Ladder 1 kb Gel 2 (PCR) Minipreps from yesterday (04/05) 17, 18 Pss/sLac/LuxI III, 2 cultures 19 Pms/sTet/LuxI Digestion from today(see further) 19 Pms/sTet/LuxI → Colonies 7, 9, 10, 17, 18, 19 seem to be of a right size Restriction digest and gel electrophoresis of yesterday's minipreps with E+P Gel 1 1 attP/PmsRFP, col 1 Ladder 1kb 2 attP/PmsRFP, col 2 - 3 attP/PsmRFP, col 1 - 4 attP/PsmRFP, col 2 - 5 attP/PssRFP, col 1 - 6 attP/PssRFP, col 2 - 7 Pms/mTet/LuxI, col 1 - 8 Pms/mTet/LuxI, col 2 - 9 Pss/wTet/LuxI, col 1 Gel 2 10 Pss/wTet/LuxI, col 2 - 11 Psm/sTet/LuxI, col 1 - 12 Psm/sTet/LuxI, col 2 - 13 Psm/wTetIII/I13507/pLux/GFP, col 1 - 14 Psm/wTetIII/I13507/pLux/GFP, col 1 - 15 Psm/wTetII/I13507/pLux/GFP, col 1 - 16 Psm/wTetII/I13507/pLux/GFP, col 2 - 17 Pss/sLac/LuxI III, col 1 - 18 Pss/sLac/LuxI III, col 2 19 Pms/sTet/LuxI (on the PCR gel, see above) → Digestions 1, 2, 7, 9, 10, 17, 18, 19 seem to be of a right size