IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/09/04

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Raphaël
Minipreps and glycerols

  • attP/Pms/attC/RFP
  • attP/Psm/attC/RFP
  • attP/Pss/attC/RFP
  • Pms/mTet/LuxI
  • Pss/wTet/LuxI
  • Psm/sTet/LuxI
  • Psm/wTetIII/I13507/pLux/GFP
  • Psm/wTetII/I13507/pLux/GFP
  • Pss/sLac/LuxI III
  • Pms/sTet/LuxI


Integration into the chromosome (Int lambda protocol)

  • attP/Pms/attC/RFP
  • attP/Psm/attC/RFP
  • attP/Pss/attC/RFP


Overnight cultures

  • PswLac, 4 colonies (for characterisation)
  • PssLac, 1 colony (for characterisation)