IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/10/02

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Entry title

  • K12Z1; DH5alpha; DB3.1 were revived.
  • Ligation of
  • J23119(S/P)+ [S+I7](PCR purified)(X/P);
  • R0010(S/P)+ [S+I7](PCR purified)(X/P);
  • Both the above ligations were Transformed as well as the controls C1 to C8.
  • Miniprep of J+I7(plate2) and J+E0420(Plate2).
  • J24674 and J14676 were also transformed.