IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/10/01
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Competent cells could not be prepared cause OD of cells shot up.
inoculated J23119 + E0420 and J23119 + I13507.
Inoculated DH5alpha for competent cell prparation.
Transformation of:
J23119 (amp and Chl)
The controls C1 to C8 which did not work the other day due to contamination.
J24674 from kit (plate 2; 9D);
J24676 from kit (plate2; 5O)
J23119 S/P digested and alkaline phosphatased.
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