IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/28

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Entry title

  • Miniprep performed on the part J13002 which was inoculated the earlier night.
  • Decision to repeat all the constructions using 2A assembly.
  • The following parts were digested
  • J23119 (S/P)
  • J23119 (S/P)
  • R0010 (S/P)
  • K117005 (S/P)
  • B0014 (E/X)
  • S03879 (X/P)
  • E0420 (X/P)
  • E0420 (X/P)
  • K145151 (X/P)
  • K145151 (E/S)
  • These digestions were done with the purpose of constructing the following composite parts:
  • J23119 + S03879 -----> 001
  • K145151 + B0014 -----> 018
  • J23119 + E0420 ------> 019
  • R0010 + E0420 -------> 502
  • K117005 + K145151 ---> 503
  • Gel Order:
  • Row 1: J23119; J23119(S/P); R0010; R0010(S/P); K117005; K117005(S/P); B0014; B0014(E/X); LADDER; S03879; S03879(X/P); E0420; E0420(X/P); K145151; K145151(X/P); K145151(E/S)
  • All digestions worked except for E0420.