IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/28

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Entry title Miniprep performed on the part J13002 which was inoculated the earlier night. Decision to repeat all the constructions using 2A assembly. The following parts were digested J23119 (S/P) J23119 (S/P) R0010 (S/P) K117005 (S/P) B0014 (E/X) S03879 (X/P) E0420 (X/P) E0420 (X/P) K145151 (X/P) K145151 (E/S) These digestions were done with the purpose of constructing the following composite parts: J23119 + S03879 -----> 001 K145151 + B0014 -----> 018 J23119 + E0420 ------> 019 R0010 + E0420 -------> 502 K117005 + K145151 ---> 503 Gel Order: Row 1: J23119; J23119(S/P); R0010; R0010(S/P); K117005; K117005(S/P); B0014; B0014(E/X); LADDER; S03879; S03879(X/P); E0420; E0420(X/P); K145151; K145151(X/P); K145151(E/S) All digestions worked except for E0420.