IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/27

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  • Miniprepped the inoculated samples of J04450, J23119, I13521, J13002, with a HP1 solution without the RNAse in it - courtesy Bawla.
  • Digested the miniprepped samples and J+S1(1,1), J+S1(1,2), J+S1(1,3), J+S2(2,1), J+S2(2,2), J+S2(2,3) with E/P. buffer4, 40 mins at 37C and 20 mins at 80C
  • Gel ran all the digested samples and yesterday's PCR products
  • ROW1  : J+S2(1,1) J+S2(1,2) J+S2(1,3) J+S2(2,1) J+S2(2,2) J+S2(2,3) J+S1(1,1) J+S1(1,2) J+S1(1,3) J+S1(2,1) J+S1(2,2) J+S2(2,3) ladder J13002(1) J13002(2) J23119(1) J23119(2) J04450(1) J04450(2)
  • ROW2  : Ladder I13521(1) I13521(2) PCR1 PCR2 PCR3 NIL
  • All wells except J13002; J+S1 and J+S2 showed good DNA.
  • Transformed J13002.
  • Inoculated J13002 from already transformed colonies.