IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/29

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Entry title Repeated the Digestion of E0420. It worked fine. Did the Ligation of the composite parts as follows: J23119 + S03879 ----> 001 K145151 + B0014 ----> 018 J23119 + E0420 -----> 019 R0010 + E0420 ------> 502 K117005 + K145151 --> 503 Transformed all these parts in DH5a cells.