IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/26

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Entry title

  • Inoculations of J04450; J23119; I13521 and J13002 (duplicates of each)
  • Preparation of Amp stock.
  • PCR for
  • J23119 + S03879 (1,1)
  • J23119 + S03879 (2,2)
  • J23119
  • Gel run order:
  • J+S1(intact plasmid); J+S2(intact plasmid); Ladder; J+S1(PCR product); J+S2(PCR product); J23119(PCR product).
  • J+S1 and J+S2 - intact plasmids were fine; PCR did not work. J23119 was fine.